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KMID : 0861020110260040067
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.67 ~ p.74
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Neuroprotective effects of Angelicae Acutilobae Radix water extract against ischemia·reperfusion-induced apoptosis in SK-N-SH neuronal cells
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Oh Tae-Woo
Park Ki-Ho
Lee Mi-Young
Choi Go-Ya
Park Yong-Ki
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Abstract
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Objectives: The purpose of the study is to determine the neuroprotective effects of the water extract of
Angelicae Acutilobae Radix(AA) on ischemia·reperfusion-induced apoptosis in SK-N-SH human brain
neuronal cells.
Methods: SK-N-SH cells were treated with different concentrations of AA water extract (0.1, 0.2, 0.5 and 1.0
mg/ml) for 2 hr and then stimulated with Dulbecco’s phosphate-buffered saline containing CI-DPBS： 3mM
sodium azide and 10 mM 2-deoxy-D-glucose for 45 min, reperfused with growth medium, and incubated for
24 h. Cell viability was determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio
assay kit. The levels of caspase-3 protein were determined by Western blot and apoptotic body was observed by
Hoechst 33258 staining.
Results: AA extract significantly inhibited decreasing the cell viability in ischemia-induced SK-N-SH cells. AA
also increased the ratio of ADP/ATP in ischemia-induced neuronal cells and decreased the expression levels of
apoptotic protein, caspase-3 and apoptotic DNA damage.
Conclusions: Our results suggest that AA extract has a neuroprotective property via suppressing the apoptosis
and increasing the energy levels in neuronal cells, suggesting that AA extract may has a therapeutic potential
in the treatment of ischemic brain injury.
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KEYWORD
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Angelica acutiloba, ischemic injury, apoptosis, SK-N-SH neuroblastoma cell, neuroprotective effect
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